幸运11选5

接待您拜候武汉艾美捷科技无限公司官方网站!
站点舆图 办事热线:400-6800-868
  • 产物
  • 文章

PRODUCT CENTER

特点产物

以后地位:幸运11选5 > 特点产物

早期氧化卵白产物(AOPP)阳性规范品&试剂盒

宣布者:艾美捷科技    宣布时候:2021-06-15     
分享到:

早期氧化卵白产物.jpg


早期氧(yang)化卵白产物(AOPP,Advanced Oxidation Protein Products)是血浆卵白被(bei)体内活性氧(yang)(ROS)进犯后构成的氧(yang)化润色产物,是早先(xian)发明的一种具备促炎(yan)活性氧(yang)化应激目标。

 

1996年Witko-Sarsat等(deng)发明(ming)CRF病人血浆(jiang)中(zhong)AOPP程度较着增高(gao),首(shou)要存在(zai)于白(bai)卵(luan)白(bai)中(zhong),特点是(shi)酸性(xing)前提下(xia)在(zai)340nm有(you)特异接(jie)收峰。在(zai)体外用次(ci)氯酸(HOC1)与白(bai)卵(luan)白(bai)感化(hua)(hua)可获得(de)与CRF病人血浆(jiang)中(zhong)类似的(de)AOPP。中(zhong)性(xing)粒细胞活化(hua)(hua)时髓过氧(yang)化(hua)(hua)物酶(mei)(MPO)催化(hua)(hua)产生的(de)HOC1能够润色血浆(jiang)卵(luan)白(bai)而天生适(shi)量AOPP。AOPP在(zai)泌尿、呼(hu)吸、神(shen)经结缔(di)构造、妇科和(he)消(xiao)化(hua)(hua)等(deng)满身多个体系相干疾病产生、成长(zhang)中(zhong)的(de)关头感化(hua)(hua)均已被(bei)证(zheng)明(ming),其在(zai)浩繁疾病中(zhong)的(de)病发机制也逐步了(le)了(le)。

 

作为专业的性命迷信医药质料供给商,Cell Biolabs中国区金牌代办署理,艾美捷科技为您保举:早期氧化卵白产物(AOPP)阳性规范品&试剂盒

【1】规范品AOPP-HSA阳性规范品,AOPP-Human Serum Albumin
(早期氧化卵白产物-人血洁白卵白)
货号STA-319 (50ul, 7.5 mg/mL AOPP-HSA)
保管前提冰袋运输;收到货后,分装并保管在-20℃,以防止屡次冷冻/冻结轮回。
【2】试剂盒早期氧化卵白产物(AOPP)检测试剂盒
OxiSelect AOPP Assay Kit
货号STA-318 (200assays)
检测方式比色法
检测规模5 - 100 uM
申明书下载
尝试道理未知的含AOPP样品或氯胺规范品起首与起头显色进程的测定反映激发剂夹杂。长久孵育后,插手停止溶液,样品和规范品能够用规范比色板读取器读取。未知样品中的AOPP含量是经由过程与预约的氯胺规范曲线停止比拟来肯定的。
试剂盒组分#第一局部#
1. Chloramine Standard
2. 2Chloramine Reaction Initiator
3. Stop Solution
4. 10X Assay Diluent
#第二局部#
1. AOPP-HSA Positive Control
保管前提收到后,将AOPP-HSA阳性规范品分装并保管在-20℃,以防止屡次冷冻/冻结轮回。将一切其余试剂盒成份保管在4℃。

* 本产物仅合用于科研用处.

 

FAQ罕见题目:

1.试剂盒合(he)用于哪些(xie)范例的(de)样品?

咱(zan)们的早期氧(yang)化卵白产物(wu)(AOPP)检(jian)测试剂盒可用于(yu)检(jian)测高于(yu)检(jian)测限以(yi)上的任(ren)何样品范(fan)例,比(bi)方血清,血浆,细胞(bao)/构造裂解液(冻(dong)存样品:需在-80℃保管1年(nian)之(zhi)内(nei))。

 

2.应(ying)当若何筹办裂解(jie)液样本(ben)?

咱(zan)们倡(chang)议在含有卵白(bai)酶按捺剂的1XPBS中经由过程(cheng)超声(sheng)或匀浆裂(lie)(lie)解(jie)细胞或构造(zao),以12000g离心10分钟,而后搜集上清(qing)液(ye)作(zuo)为(wei)裂(lie)(lie)解(jie)液(ye)。咱(zan)们只保举利用(yong)PBS制备(bei)裂(lie)(lie)解(jie)液(ye),由于咱(zan)们不晓(xiao)得裂(lie)(lie)解(jie)缓冲液(ye)是不是会破坏AOPP。卵白(bai)质浓(nong)度应经由过程(cheng)卵白(bai)质测定(ding)法肯定(ding),比方BCA或Bradford。卵白(bai)质浓(nong)度将取决于样(yang)品(pin)(pin),但倡(chang)议在运(yun)转尝试之前停止样(yang)品(pin)(pin)滴定(ding)以肯定(ding)任(ren)何须要的浓(nong)缩度,以便样(yang)品(pin)(pin)落在规范(fan)曲(qu)线内。

 

3.早期氧化卵白产(chan)物(AOPP)浓度若何展现(xian)成果?

样品 AOPP 的浓度以氯胺单(dan)元 (uM) 表现 (可参(can)考(kao)下方颁发(fa)文献)。

 

成果展现:

AOPP试剂盒规范曲线.jpg

AOPP-HSA阳性规范品.jpg

AOPP试剂盒规范曲线AOPP-HSA阳性规范品

 

颁发文章:

  • Nukala, S.B. et al. (2021). Protein network analyses of pulmonary endothelial cells in chronic thromboembolic pulmonary hypertension. Sci Rep. 11(1):5583. doi: 10.1038/s41598-021-85004-z (#STA-318).

  • AlMarabeh, S. et al. (2020). Chronic intermittent hypoxia impairs diuretic and natriuretic responses to volume expansion in rats with preserved low-pressure baroreflex control of the kidney. Am J Physiol Renal Physiol. doi: 10.1152/ajprenal.00377.2020 (#STA-318).

  • Xiao, L.L. et al. (2020). Using advanced oxidation protein products and ischaemia-modified albumin to monitor oxidative stress levels in patients with drug-induced liver injury. Sci Rep. 10(1):18128. doi: 10.1038/s41598-020-75141-2 (STA-318).

  • Bloomer, R.J. et al. (2020). Meta- bolic Health Outcomes Following Nine Months of Mild Caloric Restriction in Male Rats Adhering To a Western or Vegan Diet. J Altern Complement Integr Med. 6:097. doi: 10.24966/ACIM-7562/100097 (#STA-318).

  • Bigazzi, R. et al. (2019). Hypertension in High School Students: Genetic and Environmental Factors: The HYGEF Study. Hypertension. 75(1):71-78. doi: 10.1161/HYPERTENSIONAHA.119.13818 (#STA-318).

  • McAllister, M.J. et al. (2019). Acute coffee ingestion with and without medium chain triglycerides decreases blood oxidative stress markers and increases ketone levels. Can J Physiol Pharmacol. doi: 10.1139/cjpp-2019-0458 (#STA-318).

  • Ungurianu, A. et al. (2019). Preclinical and clinical results regarding the effects of a plant-based antidiabetic formulation versus well established antidiabetic molecules. Pharmacol Res. 150:104522. doi: 10.1016/j.phrs.2019.104522 (#STA-318).

  • Smith, C. et al. (2019). Chronic testosterone deprivation sensitizes the middle-aged rat brain to damaging effects of testosterone replacement. Neuroendocrinology. doi: 10.1159/000504445 (#STA-318).

  • Gryszczyńska, B. et al. (2019). Advanced Oxidation Protein Products and Carbonylated Proteins Levels in Endovascular and Open Repair of an Abdominal Aortic Aneurysm: The Effect of Pre-, Intra-, and Postoperative Treatment. BioMed Research International. 2019(7976043)1-9 pages. doi: 10.1155/2019/7976043 (#STA-318).

  • Morsy, M.D. et al. (2019). Protective effect of combined melatonin and α-tocopherol administration in spinal cord ischemia-reperfusion injury in rat. Int. J. Morphol. 37(2):428-437. doi: 10.4067/S0717-95022019000200428 (#STA-318).

  • Albatayneh, E.M. et al. (2019). Serum Oxidative-Antioxidative Status in Patients With Alkaptonuria. J Clin Med Res. 11(5):337-344. doi: 10.14740/jocmr3801 (#STA-318).

  • Shell, B. et al. (2019). Angiotensin Type 1a Receptors in the Median Preoptic Nucleus Support Intermittent Hypoxia-Induced Hypertension. Am J Physiol Regul Integr Comp Physiol. doi: 10.1152/ajpregu.00393.2018 (#STA-318).

  • Bloomer, R. et al. (2018) Chronic Marijuana Smoking Does Not Negatively Impact Select Blood Oxidative Stress Biomarkers in Young, Physically Active Men and Women. Health. 10:960-970. doi: 10.4236/health.2018.107071 (#STA-318).

  • Sighinolfi, G. et al. (2018). AB0760 Advanced oxidation protein products in serum of patients with systemic sclerosis: a possible indicator of clinical evolution. Annals of the Rheumatic Diseases. 77:1516. doi: 10.1136/annrheumdis-2018-eular.6809 (#STA-318).

  • Wilson, E.N. et al. (2018). Chronic intermittent hypoxia induces hormonal and male sexual behavioral changes: Hypoxia as an advancer of aging. Physiol Behav. 189:64-73. doi: 10.1016/j.physbeh.2018.03.007 (#STA-318).

  • Gradinaru, D. et al. (2018). Insulin-Leptin Axis, Cardiometabolic Risk and Oxidative Stress in Elderly with Metabolic Syndrome. Exp Clin Endocrinol Diabetes. doi: 10.1055/s-0043-123825 (#STA-318).

  • Hány?ová, S. et al. (2017). Elevated plasma levels of advanced oxidation protein products in Slovak multiple sclerosis patients: possible association with different disability states. Act Nerv Super Rediviva. 59(2): 45–50 (#STA-318).

  • Sun, S. et al. (2018). Advanced oxidation protein products induce S-phase arrest of hepatocytes via the ROS-dependent, β-catenin-CDK2-mediated pathway. Redox Biol. 14:338-353. doi: 10.1016/j.redox.2017.09.011 (#STA-318).

  • Snyder, B. et al. (2017). Chronic intermittent hypoxia induces oxidative stress and inflammation in brain regions associated with early-stage neurodegeneration. Physiol. Rep. doi: 10.14814/phy2.13258 (#STA-318).

  • Budzyń, M. et al. (2017). The Association of Serum Thrombomodulin with Endothelial Injuring Factors in Abdominal Aortic Aneurysm. Hindawi BioMed Research International. doi: 10.1155/2017/2791082 (#STA-318).

  • Wan, X. et al. (2016). SIRT1-PGC1a-NFkB pathway of oxidative and inflammatory stress during Trypanosoma cruzi infection: benefits of SIRT1-targeted therapy in improving heart function in Chagas disease. PLoS Pathog. doi: 10.1371/journal.ppat.1005954 (#STA-318).

  • Gradinaru, D. et al. (2016). Adiponectin: possible link between metabolic stress and oxidative stress in the elderly. Aging Clin Exp Res. doi:10.1007/s40520-016-0629-z (#STA-318).

  • Crone, L. B. et al. (2016). Impact of meal ingestion rate and caffeine coingestion on postprandial lipemia and oxidative stress following high-fat meal consumption. J Caffeine Res. doi:10.1089/jcr.2016.0004 (#STA-318).

  • Huemer, M. et al. (2016). Clinical phenotype, biochemical profile, and treatment in 19 patients with arginase 1 deficiency. J Inherit Metab Dis. doi:10.1007/s10545-016-9928-y (#STA-318).

  • Martins, L. S. et al. (2015). Advanced Glycation Endproducts (AGE) evolution after pancreas-kidney transplantation: plasmatic and cutaneous assessments. Oxid Med Cell Longev. 2189582 (#STA-318).

  • ?ari?, B. et al. (2015). Oxidative stress impact on growth hormone secretion in the eye. Croat Med J. 56:326-333 (#STA-318).

  • Bloomer, R. J. et al. (2015). Comparison of a restricted and unrestricted vegan diet plan with a restricted omnivorous diet plan on health-specific measures. Healthcare.3:544-555 (#STA-318).

  • Jung, E. et al. (2015). Gemigliptin improves renal function and attenuates podocyte injury in mice with diabetic nephropathy. Eur J Pharmacol. doi: 10.1016/j.ejphar.2015.04.055 (#STA-318).

  • Thurmond, P. et al. (2015). Structural modifications of the prostate in hypoxia, oxidative stress, and chronic ischemia. Korean J Urol. 56:187-196 (#STA-318)..

  • Xie, Z. X. et al. (2014). Effect of GABA on oxidative stress in the skeletal muscles and plasma free amino acids in mice fed high-fat diet. J Anim Physiol Anim Nutr (Berl). doi: 10.1111/jpn.12254 (#STA-318).

 

【传递门】AGE-BSA—最好的早期糖基化终末产物(AGEs)规范品 yyds

................................................................................................

看到(dao)这儿(er),您心动了(le)吗(ma)?顿时接(jie)洽小艾吧!

或(huo)扫描下方二维(wei)码,便可接洽您的专属(shu)客服哦(e)~

 

 

作为一(yi)家具备(bei)高(gao)真(zhen)个手艺(yi)气力、进步前(qian)辈的(de)运营办(ban)理(li)(li)程度和完美(mei)的(de)市场发卖体系的(de)生物(wu)高(gao)科(ke)技企业(ye),总部位于武汉光谷高(gao)新手艺(yi)开辟(pi)区,办(ban)事(shi)面向(xiang)天下。艾美(mei)捷科(ke)技是(shi)集入口(kou)试(shi)剂、尝试(shi)室(shi)耗材发卖、手艺(yi)办(ban)事(shi)与(yu)合约开辟(pi)为一(yi)体的(de)专业(ye)化高(gao)科(ke)技公司,为用户供(gong)给(ji)专业(ye)的(de)前(qian)沿资讯、完整的(de)产物(wu)、整合的(de)处(chu)理(li)(li)计(ji)划,及(ji)优(you)良的(de)物(wu)流办(ban)事(shi)。

 

艾(ai)美捷科技与国际外优异的(de)生(sheng)物试剂供给商优坚持(chi)着紧密亲密的(de)协作干(gan)系,今朝已成为(wei)浩(hao)繁(fan)国际着名品牌的(de)中国总代(dai)办(ban)署(shu)理(li)或(huo)一级(ji)代(dai)办(ban)署(shu)理(li),首要包含:AmyJet、AATBioquest、Abbexa、Abnova、Agrisera、Anogen、Biosensis、Biovision、BioVendor、CaissonLabs、CellBiolabs、Cytoskeleton、Demeditec、Duchefa、Epigentek、Equitech-Bio、EXBio、Fitzgerald、GeneCopoeia、HycultBiotech、ImmunoReagents、ImmunoStep、Jackson、LifeSensors、LigaTrap、Lumiprobe、Mabtech、Matreya、NorgenBiotek、Origene、ProImmune、ProSpec、ScyTek、SolisBioDyne、SouthernBiotech、StressMarq、SySy、USBiological、TRC等,能够(gou)在短(duan)时候内为(wei)用户供给专业的(de)前沿资讯、完整(zheng)的(de)产物及物流办(ban)事(shi)。

产物中间分类
比来促销 MORE
特点产物 MORE
现货产物 MORE
  • 高品德保证 进步前辈(bei)的出产研发手艺
  • 高性价比 价宜质优,性(xing)价比高
  • 100%保证 从采(cai)办到利用(yong),安心无(wu)忧
  • 宁静运输 完美(mei)的掩护办法宁静(jing)运输

微信扫码在线(xian)客服

微信征(zheng)询

天下收费手(shou)艺(yi)撑持

400-6800-868

在(zai)线(xian)客服